58 research outputs found

    A novel bearing multi-fault diagnosis approach based on weighted permutation entropy and an improved SVM ensemble classifier

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    Timely and accurate state detection and fault diagnosis of rolling element bearings are very critical to ensuring the reliability of rotating machinery. This paper proposes a novel method of rolling bearing fault diagnosis based on a combination of ensemble empirical mode decomposition (EEMD), weighted permutation entropy (WPE) and an improved support vector machine (SVM) ensemble classifier. A hybrid voting (HV) strategy that combines SVM-based classifiers and cloud similarity measurement (CSM) was employed to improve the classification accuracy. First, the WPE value of the bearing vibration signal was calculated to detect the fault. Secondly, if a bearing fault occurred, the vibration signal was decomposed into a set of intrinsic mode functions (IMFs) by EEMD. The WPE values of the first several IMFs were calculated to form the fault feature vectors. Then, the SVM ensemble classifier was composed of binary SVM and the HV strategy to identify the bearing multi-fault types. Finally, the proposed model was fully evaluated by experiments and comparative studies. The results demonstrate that the proposed method can effectively detect bearing faults and maintain a high accuracy rate of fault recognition when a small number of training samples are available

    LATFormer: Locality-Aware Point-View Fusion Transformer for 3D Shape Recognition

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    Recently, 3D shape understanding has achieved significant progress due to the advances of deep learning models on various data formats like images, voxels, and point clouds. Among them, point clouds and multi-view images are two complementary modalities of 3D objects and learning representations by fusing both of them has been proven to be fairly effective. While prior works typically focus on exploiting global features of the two modalities, herein we argue that more discriminative features can be derived by modeling ``where to fuse''. To investigate this, we propose a novel Locality-Aware Point-View Fusion Transformer (LATFormer) for 3D shape retrieval and classification. The core component of LATFormer is a module named Locality-Aware Fusion (LAF) which integrates the local features of correlated regions across the two modalities based on the co-occurrence scores. We further propose to filter out scores with low values to obtain salient local co-occurring regions, which reduces redundancy for the fusion process. In our LATFormer, we utilize the LAF module to fuse the multi-scale features of the two modalities both bidirectionally and hierarchically to obtain more informative features. Comprehensive experiments on four popular 3D shape benchmarks covering 3D object retrieval and classification validate its effectiveness

    Virus-induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening

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    Plant virus technology, in particular virus-induced gene silencing, is a widely used reverse- and forward-genetics tool in plant functional genomics. However the potential of virus technology to express genes to induce phenotypes or to complement mutants in order to understand the function of plant genes is not well documented. Here we exploit Potato virus X as a tool for virus-induced gene complementation (VIGC). Using VIGC in tomato, we demonstrated that ectopic viral expression of LeMADS-RIN, which encodes a MADS-box transcription factor (TF), resulted in functional complementation of the non-ripening rin mutant phenotype and caused fruits to ripen. Comparative gene expression analysis indicated that LeMADS-RIN up-regulated expression of the SBP-box (SQUAMOSA promoter binding protein-like) gene LeSPL-CNR, but down-regulated the expression of LeHB-1, an HD-Zip homeobox TF gene. Our data support the hypothesis that a transcriptional network may exist among key TFs in the modulation of fruit ripening in tomato

    On the low frequency electromagnetic waves in quasi-two-dimensional metals

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    Here, we theoretically analyze spectra of weakly attenuated electromagnetic waves which may appear in a Fermi-liquid of charge carriers in quasi-two-dimensional (Q2D) layered conductors when an external magnetic field is applied perpendicularly to the conducting layers. We study transverse modes propagating along the magnetic field. The frequencies of the modes are assumed to be lower than the cyclotron frequency of the charge carriers. It is shown that Fermi-liquid interaction of the charge carriers in Q2D conductors gives rise to a mode which cannot appear in a gase of charged quasiparticles, as well as it happens in conventional metals. Also, we show that the Fermi surface plofile may cause significant changes in the waves spectra and we analyze these changes.Comment: 4 pages, 2 figures, accepted in Solid State Com

    Generation and analysis of recombinant Bunyamwera orthobunyaviruses expressing V5 epitope-tagged L proteins

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    The L protein of Bunyamwera virus (BUNV; family Bunyaviridae) is an RNA-dependent RNA polymerase, 2238 aa in length, that catalyses transcription and replication of the negative-sense, tripartite RNA genome. To learn more about the molecular interactions of the L protein and to monitor its intracellular distribution we inserted a 14 aa V5 epitope derived from parainfluenza virus type 5, against which high-affinity antibodies are available, into different regions of the protein. Insertion of the epitope at positions 1935 or 2046 resulted in recombinant L proteins that retained functionality in a minireplicon assay. Two viable recombinant viruses, rBUNL4V5 and rBUNL5V5, expressing the tagged L protein were rescued by reverse genetics, and characterized with respect to their plaque size, growth kinetics and protein synthesis profile. The recombinant viruses behaved similarly to wild-type (wt) BUNV in BHK-21 cells, but formed smaller plaques and grew to lower titres in Vero E6 cells compared with wt BUNV. Immunofluorescent staining of infected cells showed the L protein to have a punctate to reticular distribution in the cytoplasm, and cell fractionation studies indicated that the L protein was present in both soluble and microsomal fractions. Co-immunoprecipitation and confocal microscopic assays confirmed an interaction between BUNV L and N proteins. The recombinant viruses expressing tagged L protein will be highly valuable reagents for the detailed dissection of the role of the BUNV L protein in virus replication

    Optical Properties of Gyroid Structured Materials: From Photonic Crystals to Metamaterials

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    The gyroid is a continuous and triply periodic cubic morphology which possesses a constant mean curvature surface across a range of volumetric ll fractions. Found in a variety of natural and synthetic systems which form through self-assembly, from buttery wing scales to block copolymers, the gyroid also exhibits an inherent chirality not observed in any other similar morphologies. These unique geometrical properties impart to gyroid structured materials a host of interesting optical properties. Depending on the length scale on which the constituent materials are organised, these properties arise from starkly di erent physical mechanisms (such as a complete photonic band gap for photonic crystals and a greatly depressed plasma frequency for optical metamaterials). This article reviews the theoretical predictions and experimental observations of the optical properties of two fundamental classes of gyroid structured materials: photonic crystals (wavelength scale) and metamaterials (subwavelength scale).This work was supported by the EPSRC through the Cambridge NanoDTC EP/G037221/1, EP/G060649/1, EP/L027151/1, and ERC LINASS 320503.This is the accepted manuscript version of the article. The final version is available from Wiley via http://dx.doi.org/10.1002/adom.20140033

    Cassava genome from a wild ancestor to cultivated varieties

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    Cassava is a major tropical food crop in the Euphorbiaceae family that has high carbohydrate production potential and adaptability to diverse environments. Here we present the draft genome sequences of a wild ancestor and a domesticated variety of cassava and comparative analyses with a partial inbred line. We identify 1,584 and 1,678 gene models specific to the wild and domesticated varieties, respectively, and discover high heterozygosity and millions of single-nucleotide variations. Our analyses reveal that genes involved in photosynthesis, starch accumulation and abiotic stresses have been positively selected, whereas those involved in cell wall biosynthesis and secondary metabolism, including cyanogenic glucoside formation, have been negatively selected in the cultivated varieties, reflecting the result of natural selection and domestication. Differences in microRNA genes and retrotransposon regulation could partly explain an increased carbon flux towards starch accumulation and reduced cyanogenic glucoside accumulation in domesticated cassava. These results may contribute to genetic improvement of cassava through better understanding of its biology
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